circRNA basic information
circBase ID: hsa_circ_0025033
Name: hsa_circ_FOXM1
Synonym: circ-FOXM1
Host Gene: FOXM1
Genomic location(hg19): chr12:2966846-2983691:-
Genomic location(hg38): chr12:2857680-2874525:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0009807
MONDO name: osteosarcoma
Disease details: Osteosarcoma / OS
Disease DO ID:
3347
Disease MeSH ID:
D012516
Disease NCIt ID:
C9145
Disease ICD11 ID:
-
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor xenografts

Cell lines:

hFOB1.19; MG-63; HOS; 143B; U2OS

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: FOXM1, Ago2, beta-catenin, C-myc, MMP2, MMP9, E-cadherin, N-cadherin
Associated microRNA: miR-320a, miR-320b
Biological function: promotes proliferation, migration and EMT; inhibits apoptosis; promotes in vivo tumor growth
Molecular mechanism: Acts as a ceRNA to sponge miR-320a/miR-320b, thereby upregulating FOXM1 and activating Wnt signaling (beta-catenin nuclear localization).
Biological pathway or process:

proliferation (promotes); migration (promotes); EMT (promotes); apoptosis (inhibits); Wnt/beta-catenin (promotes)

Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Transwell Assay; TUNEL; Western Blot; IF (Immunofluorescence); In Vivo Animal Model; IHC (Immunohistochemistry)

Clinical significance:

potential biomarker for OS treatment

Description:

circ-FOXM1 is up-regulated in osteosarcoma cell lines and promotes proliferation, migration, EMT and tumor growth while suppressing apoptosis. Mechanistically, it functions as a ceRNA to sponge miR-320a/miR-320b, elevating FOXM1 and activating the Wnt/beta-catenin pathway.

Confidence score:

0.7797

Other information
Title:

Circ-FOXM1 promotes the proliferation, migration and EMT process of osteosarcoma cells through FOXM1-mediated Wnt pathway activation.

Journal: Journal of orthopaedic surgery and research
Published: 2022
PubMed ID: 35799265
Study type:

biological research

Data availability: 10.1186/s13018-022-03207-0
Code availability: -