tumor tissue; adjacent non-tumor lung tissue; NSCLC tissues; mouse tumor tissues
A549; H1299; PC9; H1650; BEAS-2B; HEK293T
cell line-derived xenograft
PI3K/AKT (promotes); PI3K/AKT/mTOR (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); glycolysis (promotes); apoptosis (inhibits); ceRNA regulation (promotes); other pathway/process (promotes)
RT-qPCR; Microarray; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Bioinformatics Analysis; Transfection; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); CCK8; Colony Formation Assay; Transwell Assay; Annexin V/PI Flow Cytometry; Flow Cytometry(Non-apoptosis/cycle); Western Blot; In Vivo Animal Model
Potential diagnostic marker and therapeutic target in NSCLC.
Hypoxia-induced circ_0017521 is up-regulated in NSCLC tissues and hypoxic NSCLC cells and functions as an oncogenic circRNA. It promotes proliferation, migration, invasion, EMT, glycolysis and xenograft tumor growth by sponging miR-532-3p to upregulate PFKFB3 and activate PI3K/AKT/mTOR signaling, while circ_0017521 knockdown increases apoptosis and suppresses malignant progression.
0.8355
Hypoxia-induced circ_0017521 enhances glycolysis and promotes NSCLC progression via upregulating the PFKFB3/PI3K-AKT pathway.
combined biological and clinical study