| Expression pattern: |
UP |
| Associated gene: |
NOTCH1 |
| Associated microRNA: |
miR-495 |
| Biological function: |
promotes angiogenesis by increasing tube formation and cell migration in endothelial cells |
| Molecular mechanism: |
circ-MBOAT2 acts as a sponge of miR-495 and regulates NOTCH1 expression, thereby promoting angiogenesis |
| Biological pathway or process: |
angiogenesis (promotes); migration (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; Transwell Assay; Wound Healing Assay; Tube Formation Assay; Western Blot; In Vivo Animal Model |
| Clinical significance: |
The expression of circ-MBOAT2 was significantly higher in patients with improved myocardial perfusion than in those patients without after revascularization of CTO; potential biomarker/prognostic factor |
| Description: |
circ-MBOAT2 is up-regulated in plasma from patients with coronary chronic total occlusion and promotes angiogenesis in endothelial cells. Mechanistically, it sponges miR-495 to increase NOTCH1 expression, enhancing tube formation and migration, and its higher level associates with myocardial perfusion improvement after CTO revascularization. |
| Confidence score: |
0.6829 |