| Expression pattern: |
UP |
| Associated gene: |
PKR, eIF2alpha, ATF4 |
| Associated microRNA: |
- |
| Biological function: |
inhibits TNBC cell proliferation; enhances ER stress and Huaier-induced immunogenic cell death; enhances tumor suppression effects of Huaier |
| Molecular mechanism: |
circCLASP1 directly binds PKR in the cytoplasm, blocks PKR degradation and stabilizes PKR, thereby activating PKR/eIF2alpha/ATF4-mediated ER stress to promote Huaier-induced ICD |
| Biological pathway or process: |
other pathway/process (promotes); apoptosis (other); proliferation (inhibits); immune regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; Bioinformatics Analysis; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RNase R Treatment; RNA Pull-Down; RIP (RNA Immunoprecipitation); FISH / smFISH; IF (Immunofluorescence); Transfection; MTT; Western Blot |
| Clinical significance: |
- |
| Description: |
circCLASP1 (hsa_circ_0002374), derived from CLASP1, is up-regulated after Huaier treatment in TNBC cells. It binds and stabilizes PKR in the cytoplasm, enhancing PKR/eIF2alpha (and ATF4) signaling to promote ER stress-associated immunogenic cell death and suppress TNBC cell proliferation. |
| Confidence score: |
0.7962 |