circRNA basic information
circBase ID: hsa_circ_0039411
Name: hsa_circ_MMP2
Synonym: -
Host Gene: MMP2
Genomic location(hg19): chr16:55523562-55540586:+
Genomic location(hg38): chr16:55489650-55506674:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005061
MONDO name: lung adenocarcinoma
Disease details: lung adenocarcinoma
Disease DO ID:
 3910
Disease MeSH ID:
 C538231
Disease NCIt ID:
 C3512
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

LUAD samples; matched para-tumor ones; tumor tissue
Cell lines:

A549; HCC827; PC-9; NCI-H1975; NCI-H1299; 16HBE
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: FOXM1, IGF2BP3, beta-catenin
Associated microRNA: -
Biological function: promotes proliferation, migration and EMT; promotes tumor growth and metastasis in vivo
Molecular mechanism: FOXM1 transcriptionally activates circ-0039411; circ-0039411 recruits IGF2BP3 to enhance FOXM1 mRNA stability, forming a positive feedback loop; ceRNA unlikely based on Ago2-RIP
Biological pathway or process:

proliferation (promotes); migration (promotes); EMT (promotes); metastasis (promotes); Wnt/beta-catenin (other); mRNA stability (promotes)
Detected method:
Q
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Nuclear-Cytoplasmic Fractionation; ChIP / ChIP-seq; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; Western Blot; IF (Immunofluorescence); EdU Staining; Colony Formation Assay; Transwell Assay; In Vivo Animal Model; IHC (Immunohistochemistry); H&E Staining; Survival Analysis; Bioinformatics Analysis
Clinical significance:

high circ-0039411 level was linked to unsatisfactory prognosis of LUAD patients
Description:

In LUAD, circ-0039411 (circ-MMP2) is up-regulated and promotes proliferation, migration, EMT, tumor growth and metastasis. FOXM1 transcriptionally activates circ-0039411, and circ-0039411 recruits IGF2BP3 to stabilize FOXM1 mRNA, forming a positive feedback loop that drives malignant progression.
Confidence score:

0.8738
Other information
Title:

Circ-MMP2 (circ-0039411) induced by FOXM1 promotes the proliferation and migration of lung adenocarcinoma cells in vitro and in vivo.

Journal: Cell death & disease
Published: 2020
PubMed ID: 32513952
Study type:

combined biological and clinical study
Data availability: Supplementary file 1; TCGA
Code availability: -