| Expression pattern: |
UP |
| Associated gene: |
KLF8, Ago2 |
| Associated microRNA: |
miR-1253 |
| Biological function: |
Promotes NSCLC cell proliferation and glycolysis and inhibits apoptosis; promotes tumor formation in vivo. |
| Molecular mechanism: |
Acts as a miR-1253 sponge to upregulate KLF8 (ceRNA mechanism), thereby promoting malignant phenotypes and glycolysis. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); glycolysis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; Colony Formation Assay; EdU Staining; Annexin V/PI Flow Cytometry; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model |
| Clinical significance: |
CircSEC24A expression was significantly associated with distant metastasis, T stage, and TNM stages of NSCLC patients. |
| Description: |
CircSEC24A is up-regulated in NSCLC tissues and cell lines and functions as an oncogenic circRNA. It sponges miR-1253 to de-repress KLF8, thereby promoting proliferation and glycolysis while inhibiting apoptosis, and its knockdown suppresses xenograft tumor growth. |
| Confidence score: |
0.7975 |