circRNA basic information
circBase ID: hsa_circ_0001451
Name: hsa_circ_FBXW7
Synonym: hsa_circ_001988
Host Gene: FBXW7
Genomic location(hg19): chr4:153332454-153333681:-
Genomic location(hg38): chr4:152411302-152412529:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0001056
MONDO name: gastric cancer
Disease details: gastric cancer
Disease DO ID:
 10534
Disease MeSH ID:
 -
Disease NCIt ID:
 C9331
Disease ICD11 ID:
 1397617262
Disease OMIM ID:
 613659
Species: Human
Species details: Homo sapiens
Tissue specimen:

primary GC samples; noncancerous tissues; GC tissues; matched noncancerous mucosa
Cell lines:

GES-1; BGC823; HGC27; SGC7901; AGS
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: FBXW7, CCDC6, yes-associated protein 1, cyclinD1, EIF4A3, U2AF65, E-cadherin, N-cadherin, Vimentin
Associated microRNA: miR-197-3p
Biological function: Hsa_circ_001988 acts as an anti-oncogenic circRNA in gastric cancer, inhibiting cell viability, proliferation, colony formation, migration, invasion, EMT and xenograft tumor growth, while inducing G1-phase arrest.
Molecular mechanism: Hsa_circ_001988 sponges miR-197-3p to promote FBXW7 expression, thereby regulating downstream cyclinD1, YAP1, CCDC6 and EMT-related proteins; EIF4A3 and U2AF65 bind hsa_circ_001988 and promote its cyclization and expression.
Biological pathway or process:

proliferation (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits); EMT (inhibits); cell cycle (other); ceRNA regulation (other); Hippo/YAP (inhibits)
Detected method:
Q
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RT-qPCR; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Transfection; CCK8; Colony Formation Assay; Cell Cycle Assay; Wound Healing Assay; Transwell Assay; In Vivo Animal Model; Western Blot; ROC Analysis; Bioinformatics Analysis
Clinical significance:

Low hsa_circ_001988 expression was associated with WHO histological types, Lauren types and tumor invasion depth; ROC analysis suggested potential diagnostic value in gastric cancer.
Description:

Hsa_circ_001988 is downregulated in gastric cancer tissues and cell lines and functions as a tumor suppressor. It inhibits GC proliferation, colony formation, migration, invasion, EMT and xenograft tumor growth through an EIF4A3/U2AF65-mediated hsa_circ_001988/miR-197-3p/FBXW7 regulatory axis. Its reduced expression is associated with clinicopathological features and it may have diagnostic value for GC.
Confidence score:

0.8006
Other information
Title:

Hsa_circ_001988 attenuates GC progression in vitro and in vivo via sponging miR-197-3p.

Journal: Journal of cellular physiology
Published: 2021
PubMed ID: 32592202
Study type:

combined biological and clinical study
Data availability: The datasets used and/or analyzed during the present study are available from the corresponding author upon reasonable request.
Code availability: -