| Expression pattern: |
UP |
| Associated gene: |
PDL1 |
| Associated microRNA: |
miR-607 |
| Biological function: |
promotes proliferation, migration, invasion, angiogenesis, immune escape, and apoptosis in NSCLC cells; promotes tumor growth in vivo |
| Molecular mechanism: |
ceRNA mechanism; circ_0101675 acts as a sponge for miR-607 to regulate PDL1 expression |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); angiogenesis (promotes); immune regulation (promotes); apoptosis (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; CCK8; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Tube Formation Assay; Annexin V/PI Flow Cytometry; ELISA; Western Blot; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Clinical Sample Validation |
| Clinical significance: |
high circ_0101675 was related to the TNM grade, lymph node metastasis and tumor size of NSCLC patients |
| Description: |
circ_0101675 is upregulated in NSCLC tissues and cells and acts as an oncogenic circRNA. It promotes proliferation, migration, invasion, angiogenesis, immune evasion, and tumor growth by sponging miR-607 and relieving repression of PDL1. |
| Confidence score: |
0.8039 |