| Expression pattern: |
UP |
| Associated gene: |
IGF2BP2, CREB1 mRNA |
| Associated microRNA: |
miR-133b |
| Biological function: |
promotes proliferation and migration; promotes colorectal tumorigenesis, growth and metastasis |
| Molecular mechanism: |
circEZH2 sponges miR-133b to upregulate IGF2BP2; circEZH2 binds IGF2BP2 and blocks ubiquitination-dependent degradation; circEZH2/IGF2BP2 enhances stability of m6A-modified CREB1 mRNA |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); metastasis (promotes); ceRNA regulation (promotes); mRNA stability (promotes); m6A modification (promotes); ubiquitination (inhibits) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; FISH / smFISH; RNA Pull-Down; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; H&E Staining; Survival Analysis; Bioinformatics Analysis; MeRIP / MeRIP-seq |
| Clinical significance: |
High circEZH2 expression was significantly associated with tumor size, tumor stage, lymph node status and distant metastasis; higher expression of circEZH2 led to a significantly poorer overall survival |
| Description: |
circEZH2 (hsa_circ_0006357) is up-regulated in colorectal cancer and predicts poor overall survival. It promotes CRC proliferation, migration and metastasis by sponging miR-133b to increase IGF2BP2, and by binding IGF2BP2 to prevent its ubiquitination-dependent degradation, thereby enhancing the stability of m6A-modified CREB1 mRNA. |
| Confidence score: |
0.8839 |