| Expression pattern: |
UP |
| Associated gene: |
CCND2, AGO2 |
| Associated microRNA: |
miR-154-5p |
| Biological function: |
Promotes gastric cancer cell growth/proliferation and survival; knockdown promotes apoptosis. |
| Molecular mechanism: |
Circ-malat1 acts as a cytoplasmic ceRNA that sponges miR-154-5p to enhance CCND2 expression. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); cell cycle (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Back-Splice Junction PCR / divergent primers PCR; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; EdU Staining; Cell Cycle Assay; Annexin V/PI Flow Cytometry; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
Circ-malat1 is up-regulated in gastric cancer cells and promotes cell growth/proliferation. It mainly localizes in the cytoplasm and functions as a ceRNA that sponges miR-154-5p, thereby increasing CCND2 expression to drive gastric carcinogenesis. |
| Confidence score: |
0.7626 |