| Expression pattern: |
UP |
| Associated gene: |
DICER, RBM3 |
| Associated microRNA: |
- |
| Biological function: |
Promotes glioblastoma stem-like cell tumorigenicity by retaining DICER in the nucleus and suppressing mature microRNAome; circ2082 knockdown mitigates tumorigenicity and improves survival in mouse xenografts. |
| Molecular mechanism: |
Forms a nuclear complex with DICER and RBM3; RBM3 binds directly to circ2082, enabling DICER nuclear retention and impaired microRNA maturation; circ2082 knockdown restores cytosolic DICER and derepresses mature microRNAome. |
| Biological pathway or process: |
ceRNA regulation (other); other pathway/process (other) |
| Detected method: |
Q
H
M
|
| Validation methods: |
RT-qPCR; Microarray; RNase R Treatment; Sanger Sequencing; RIP (RNA Immunoprecipitation); RNA Pull-Down; Western Blot; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Transfection; Colony Formation Assay; In Vivo Animal Model; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
circ2082-dependent signatures correlate with favorable outcomes in patients with glioblastoma. |
| Description: |
circ2082 (hsa_circ_0002082), derived from MALAT1, is up-regulated in glioblastoma GSCs and forms a nuclear complex with RBM3 and DICER. This complex retains DICER in the nucleus and contributes to impaired microRNA maturation and microRNAome suppression; circ2082 knockdown relocalizes DICER to the cytosol, restores the microRNAome, and reduces tumorigenicity in vitro and in xenograft models, with links to favorable patient outcomes. |
| Confidence score: |
0.8208 |