| Expression pattern: |
UP |
| Associated gene: |
AFF4, WTAP, IGF2BP3, AGO2 |
| Associated microRNA: |
miR-93-5p |
| Biological function: |
promotes osteogenic differentiation (osteogenesis) of LF cells |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-93-5p to upregulate AFF4; m6A modification increases circCDK14 stability via WTAP and IGF2BP3 |
| Biological pathway or process: |
ceRNA regulation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; Bioinformatics Analysis; RT-qPCR; Clinical Sample Validation; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Actinomycin D / DRB Stability Assay; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; RNA Pull-Down; MeRIP / MeRIP-seq; Transfection; Western Blot; IHC (Immunohistochemistry) |
| Clinical significance: |
promising therapeutic target for OLF |
| Description: |
circCDK14 is up-regulated in OLF tissues and promotes osteogenic differentiation of ligamentum flavum cells. It functions as a ceRNA by sponging miR-93-5p to increase AFF4, and its abundance is enhanced by WTAP-mediated m6A modification recognized by IGF2BP3 to improve circCDK14 stability. |
| Confidence score: |
0.869 |