circRNA basic information
circBase ID: -
Name: hsa_circ_SYPL1
Synonym: circSYPL1
Host Gene: SYPL1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma / HCC
Disease DO ID:
684, 686
Disease MeSH ID:
D006528
Disease NCIt ID:
C3099
Disease ICD11 ID:
1294035808
Disease OMIM ID:
114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

primary liver cancerous tissue; metastatic liver cancerous tissue; liver cancer tumors; adjacent healthy tissue specimens; surrounding tissues; lung tissues

Cell lines:

7402; 7721; HepG2; PLC-PRF-5; LO2; HEK 293T

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: EZH2
Associated microRNA: miR-506-3p
Biological function: Promotes HCC cell proliferation/survival and migration/invasion/metastasis; inhibits apoptosis; promotes tumor xenograft growth and lung metastasis.
Molecular mechanism: Acts as a ceRNA that sponges miR-506-3p, thereby upregulating EZH2 expression; supported by luciferase reporter and RNA pull-down assays.
Biological pathway or process:

proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (other)

Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Transfection; BrdU; CCK8; TUNEL; Transwell Assay; Luciferase Reporter Assay; RNA Pull-Down; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Bioinformatics Analysis

Clinical significance:

May be a potential diagnostic biomarker and therapeutic target for HCC.

Description:

circSYPL1 is upregulated in HCC tissues/cell lines and promotes malignant phenotypes (proliferation, invasion/metastasis) while inhibiting apoptosis. Mechanistically, it functions as a ceRNA that sponges miR-506-3p to increase EZH2 expression, and perturbing miR-506-3p or EZH2 reverses circSYPL1-driven effects.

Confidence score:

0.7552

Other information
Title:

circSYPL1 Promotes the Proliferation and Metastasis of Hepatocellular Carcinoma via the Upregulation of EZH2 Expression by Competing with hsa-miR-506-3p.

Journal: Journal of oncology
Published: 2022
PubMed ID: 35345511
Study type:

combined biological and clinical study

Data availability: The data sets analyzed or generated during the study are available from the corresponding author on reasonable request.
Code availability: -