| Expression pattern: |
UP |
| Associated gene: |
NRAS, Ago2, MEK1, ERK1/2, PCNA, MMP2 |
| Associated microRNA: |
has-miR-502-5p |
| Biological function: |
promotes tumorigenesis and metastasis; promotes proliferation; promotes invasion; promotes liver metastasis |
| Molecular mechanism: |
circDLST acts as a miRNA sponge (ceRNA) for miR-502-5p, thereby increasing NRAS and activating NRAS/MEK1/ERK1/2 signaling, with upregulation of PCNA and MMP2. |
| Biological pathway or process: |
ERK (promotes); proliferation (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
H
|
| Validation methods: |
FISH / smFISH; RT-qPCR; RNase R Treatment; Microarray; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; MTT; Colony Formation Assay; EdU Staining; Transwell Assay; Wound Healing Assay; Western Blot; In Vivo Animal Model; H&E Staining; Clinical Sample Validation; Cohort Study; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High expression of circDLST acted as an independent prognostic factor of poor survival in patients with GC. |
| Description: |
circDLST is up-regulated in gastric cancer and mainly localized in the cytoplasm. It promotes GC cell proliferation, invasion, and metastasis by sponging miR-502-5p, releasing NRAS and activating the NRAS/MEK1/ERK1/2 pathway, and its high expression predicts poor survival. |
| Confidence score: |
0.8427 |