| Expression pattern: |
UP |
| Associated gene: |
Caspase3, cyto c |
| Associated microRNA: |
hsa-miR-1202, hsa-miR-27a-3p, hsa-miR-27b-3p, hsa-miR-30d-3p, hsa-miR-628-5p |
| Biological function: |
promotes mitochondrial fission; promotes apoptosis under oxidative stress |
| Molecular mechanism: |
circSLC8A1 modulates oxidative stress-associated mitochondrial fission to affect mitochondrial apoptosis (Caspase3, cyto c); predicted circRNA-miRNA-mRNA network also reported |
| Biological pathway or process: |
apoptosis (promotes); mitochondrial function (other); other pathway/process (other) |
| Detected method: |
Q
S
|
| Validation methods: |
circRNA-seq; RNase R Treatment; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RT-qPCR; Clinical Sample Validation; ROC Analysis; Bioinformatics Analysis; Transfection; TUNEL; Western Blot; RNA-seq |
| Clinical significance: |
circSLC8A1 has the best ROC in the verification of clinical samples |
| Description: |
circSLC8A1 is up-regulated in MI peripheral blood and in an oxidative-stress AC16 injury model. Functionally, circSLC8A1 promotes mitochondrial fission and sensitizes cardiomyocytes to apoptosis under oxidative stress, with changes in Caspase3 and cyto c, and it shows strong diagnostic value by ROC analysis. |
| Confidence score: |
0.7921 |