| Expression pattern: |
UP |
| Associated gene: |
NLRP3 |
| Associated microRNA: |
miR-93-5p |
| Biological function: |
Promotes pyroptosis and inflammatory response in renal tubular epithelial cells in DKD/high-glucose condition. |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-93-5p, thereby disinhibiting NLRP3 and activating the NLRP3 inflammasome to promote pyroptosis. |
| Biological pathway or process: |
pyroptosis (promotes); inflammation (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; divergent primers PCR; Back-Splice Junction PCR; Sanger Sequencing; Luciferase Reporter Assay; Bioinformatics Analysis; Transfection; CCK8; ELISA; Western Blot; In Vivo Animal Model |
| Clinical significance: |
Potential for clinical diagnosis and treatment of DKD. |
| Description: |
circ_0004951 (circTRPM7; hsa_circ_0004951) is upregulated in DKD kidney tissues and in HK2 cells under high glucose. It promotes renal tubular cell pyroptosis and inflammation by sponging miR-93-5p, thereby increasing NLRP3 expression and activating the NLRP3 inflammasome. |
| Confidence score: |
0.7435 |