circRNA basic information
circBase ID: -
Name: hsa_circ_FOXO3
Synonym: circFOXO3
Host Gene: FOXO3
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma / HCC
Disease DO ID:
 684, 686
Disease MeSH ID:
 D006528
Disease NCIt ID:
 C3099
Disease ICD11 ID:
 1294035808
Disease OMIM ID:
 114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

-
Cell lines:

BEL-7404; Hep G2; Hep 3B2.1-7; HuH-7; Li-7; HHL-5
In vivo animal model:

-
circRNA-disease information
Expression pattern:
DN
Associated gene: PI3K, p-PI3K, Akt, p-Akt, Caspase-3, Bcl-2, Bax, MMP-2, MMP-9
Associated microRNA: -
Biological function: Inhibits proliferation, invasion, and migration; promotes apoptosis in hepatocellular carcinoma cells.
Molecular mechanism: Overexpression of circFOXO3 inhibits PI3K/Akt pathway activation (reduced phosphorylation of PI3K and Akt), thereby suppressing malignant phenotypes.
Biological pathway or process:

PI3K/AKT (inhibits); proliferation (inhibits); invasion (inhibits); migration (inhibits); apoptosis (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Transfection; CCK8; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; Western Blot; Clinical Sample Validation
Clinical significance:

-
Description:

circFOXO3 is down-regulated in hepatocellular carcinoma cell lines compared with normal hepatocytes. Forced overexpression of circFOXO3 in HuH-7 cells suppresses viability/proliferation, invasion and migration, and increases apoptosis, at least partly by inhibiting PI3K/Akt signaling (reduced p-PI3K and p-Akt).
Confidence score:

0.5434
Other information
Title:

Effects of circFOXO3 on the Proliferation and Invasion of Liver Cancer Cells by Regulating PI3K/Akt Pathway.

Journal: Contrast media & molecular imaging
Published: 2022
PubMed ID: 35909586
Study type:

biological research
Data availability: The data generated or analyzed during this study are included within the article.
Code availability: -