| Expression pattern: |
UP |
| Associated gene: |
AKT3 |
| Associated microRNA: |
miR-217 |
| Biological function: |
Circ_0000144 promotes TC cell proliferation, viability, migration, invasion and EMT; circ_0000144 knockdown represses malignant biological behaviors of TC cells. |
| Molecular mechanism: |
Circ_0000144 acts through the miR-217/AKT3 pathway, targeting miR-217 and up-regulating AKT3 expression to promote TC progression. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); ceRNA regulation (promotes); PI3K/AKT (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; CCK8; BrdU; Transwell Assay; Western Blot; Luciferase Reporter Assay; Bioinformatics Analysis |
| Clinical significance: |
High circ_0000144 expression was related to tumor size, TNM stage and lymph node metastasis, and circ_0000144 could be a potential biomarker and therapy target for TC. |
| Description: |
Circ_0000144 is up-regulated in thyroid carcinoma tissues and cell lines, and its high expression is associated with larger tumor size, advanced TNM stage and lymph node metastasis. Functionally, circ_0000144 promotes TC cell proliferation, migration, invasion and EMT. Mechanistically, it targets miR-217 and up-regulates AKT3 expression, thereby facilitating TC progression through the miR-217/AKT3 pathway. |
| Confidence score: |
0.6521 |