| Expression pattern: |
UP |
| Associated gene: |
GNB1, POLA2 |
| Associated microRNA: |
miR-326 |
| Biological function: |
Promotes lung cancer cell stemness, including sphere-formation ability, ALDH1 activity, and stemness marker expression; promotes lung cancer progression. |
| Molecular mechanism: |
circ_POLA2 functions as a ceRNA by sponging miR-326, thereby regulating the miR-326/GNB1 axis and increasing GNB1-mediated lung cancer cell stemness. |
| Biological pathway or process: |
stemness (promotes); ceRNA regulation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
H
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; FISH / smFISH; Clinical Sample Validation; Luciferase Reporter Assay; RNA Pull-Down; Transfection; CCK8; Western Blot; Cohort Study; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circ_POLA2 expression predicted poor prognosis and was positively associated with distant metastasis, advanced TNM stage, and presence of vascular of lung cancer patients. |
| Description: |
circ_POLA2 (hsa_circ_0022812), derived from POLA2, is up-regulated in lung cancer tissues and cell lines and high expression indicates poor overall survival. It promotes lung cancer cell stemness by acting as a ceRNA for miR-326, thereby regulating GNB1 expression through the circ_POLA2/miR-326/GNB1 axis. The study suggests circ_POLA2 may serve as a prognostic biomarker and therapeutic target in lung cancer. |
| Confidence score: |
0.8647 |