| Expression pattern: |
UP |
| Associated gene: |
SYP, ENO2, PSA, BRN2, PCDH11Y |
| Associated microRNA: |
- |
| Biological function: |
Promotes neuroendocrine differentiation and modulates neuroendocrine marker expression (delays ENO2 and SYP upregulation when silenced; increases PSA and BRN2 when silenced; reduces linear PCDH11Y). |
| Molecular mechanism: |
RNA-interference-mediated knockdown indicates circPCDH11Y regulates transcriptional levels of neuroendocrine markers; specific downstream molecular mechanism (e.g., miRNA sponging) not defined. |
| Biological pathway or process: |
ceRNA regulation (other); proliferation (other) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; Bioinformatics Analysis; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RT-qPCR; Transfection; Flow Cytometry（Non-apoptosis/cycle）; Cell Cycle Assay |
| Clinical significance: |
Potential biomarker for early detection of t-NEPC; detectable in LNCaP-secreted extracellular vesicles post-NED, suggesting potential as a circulating biomarker (clinical validation not yet performed). |
| Description: |
circPCDH11Y is strongly up-regulated during neuroendocrine transdifferentiation of AR-dependent LNCaP prostate cancer cells and is detectable in LNCaP-derived extracellular vesicles after NED. Knockdown of circPCDH11Y modulates key NED markers (SYP, ENO2, PSA, BRN2, and linear PCDH11Y), supporting a role in promoting or timing neuroendocrine differentiation and suggesting biomarker potential for t-NEPC. |
| Confidence score: |
0.642 |