| Expression pattern: |
DN |
| Associated gene: |
PON2 |
| Associated microRNA: |
miR-30a-3p |
| Biological function: |
prevents chondrocyte apoptosis and cartilage degradation; maintains extracellular matrix (ECM) homeostasis; reduces intracellular ROS; inhibits mitochondria-mediated apoptosis |
| Molecular mechanism: |
circHIPK3 acts as a miRNA sponge for miR-30a-3p, thereby promoting PON2 expression and regulating mitochondria-mediated apoptosis |
| Biological pathway or process: |
apoptosis (inhibits); mitochondrial function (promotes); oxidative phosphorylation (other); ceRNA regulation (promotes); fibrosis (not specified) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; Bioinformatics Analysis; Transfection; Annexin V/PI Flow Cytometry; Western Blot; Flow Cytometry（Non-apoptosis/cycle）; In Vivo Animal Model; H&E Staining; TUNEL; IHC (Immunohistochemistry) |
| Clinical significance: |
targeting the circHIPK3/miR-30a-3p/PON2 axis might be a potential strategy for OA treatment |
| Description: |
circHIPK3 is down-regulated in osteoarthritis cartilage and chondrocytes. It suppresses ROS accumulation and mitochondria-mediated apoptosis and protects ECM homeostasis by sponging miR-30a-3p to maintain PON2 expression, thereby alleviating cartilage degradation in the DMM mouse model. |
| Confidence score: |
0.7981 |