| Expression pattern: |
UP |
| Associated gene: |
DHCR24 |
| Associated microRNA: |
miR-892b |
| Biological function: |
Promotes BLCA cell proliferation, migration/invasion, glycolysis, and tumor growth; knockdown suppresses these malignant phenotypes. |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-892b to increase DHCR24 expression (ceRNA-like regulation), thereby promoting malignant behaviors and glycolytic metabolism in BLCA. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); glycolysis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Luciferase Reporter Assay; RNA Pull-Down; Transfection; EdU; Colony Formation Assay; Transwell Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
circ_0003221 upregulation suggested potential as a diagnostic biomolecule and therapeutic target in BLCA. |
| Description: |
circ_0003221 is upregulated in bladder cancer and promotes proliferation, migration/invasion, glycolysis, and xenograft tumor growth. Mechanistically, it sponges miR-892b to de-repress DHCR24, forming a circ_0003221/miR-892b/DHCR24 regulatory axis. |
| Confidence score: |
0.7548 |