| Expression pattern: |
UP |
| Associated gene: |
GSK3beta |
| Associated microRNA: |
hsa-miR-1179, hsa-miR-378 g, hsa-miR-1324, hsa-miR-6757-3p, hsa-miR-29b-2-5p |
| Biological function: |
Promotes oxidative stress (increases ROS generation) in high glucose-injured HK-2 cells; involved in dapagliflozin protective effects against DKD. |
| Molecular mechanism: |
Acts in a ceRNA network: hsa_circRNA_012448 negatively regulates hsa-miR-29b-2-5p and increases GSK3beta expression, contributing to oxidative stress response. |
| Biological pathway or process: |
ceRNA regulation (promotes); oxidative stress response (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; RNase R Treatment; FISH / smFISH; Bioinformatics Analysis; Transfection; In Vivo Animal Model |
| Clinical significance: |
- |
| Description: |
In high-glucose-treated HK-2 cells (an in vitro DKD model), hsa_circRNA_012448 is up-regulated and is reduced by dapagliflozin. It functions in a ceRNA-like axis with hsa-miR-29b-2-5p and GSK3beta, promoting oxidative stress (ROS generation), suggesting a mechanism for dapagliflozin’s protective action in DKD. |
| Confidence score: |
0.5695 |