| Expression pattern: |
UP |
| Associated gene: |
ALDOA, POU5F1, OCT4, HK2, PKM2, AGO2 |
| Associated microRNA: |
miR-335 |
| Biological function: |
promotes liver metastasis and metabolic reprogramming (glucose uptake and lactate production) in breast cancer; promotes migration/invasion; supports tumor growth |
| Molecular mechanism: |
circKIF4A acts as a miRNA sponge for miR-335, thereby modulating ALDOA/OCT4 and downstream HK2/PKM2 to reprogram glucose metabolism and promote liver metastasis |
| Biological pathway or process: |
glycolysis (promotes); metastasis (promotes); migration (promotes); invasion (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; Transwell Assay; Western Blot; In Vivo Animal Model; H&E Staining |
| Clinical significance: |
- |
| Description: |
circKIF4A is up-regulated in primary breast cancer and liver metastasis tissues/cell lines and promotes metastatic behavior and glycolytic metabolic reprogramming. Mechanistically, it mainly localizes in the cytoplasm and sponges miR-335, thereby increasing ALDOA/OCT4 and downstream HK2/PKM2 expression to enhance glucose uptake and lactate production, promoting liver metastasis. |
| Confidence score: |
0.7437 |