| Expression pattern: |
UP |
| Associated gene: |
IL-23R |
| Associated microRNA: |
miR-125a-3p |
| Biological function: |
promotes the Th17 cell response; increases IL-17A expression; promotes IL-23R expression |
| Molecular mechanism: |
circNUP214 acts as a ceRNA sponge for miR-125a-3p, thereby regulating IL-23R and promoting Th17/IL-17A responses. |
| Biological pathway or process: |
immune regulation (promotes); ceRNA regulation (promotes); proliferation (other) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Transfection; Luciferase Reporter Assay; Flow Cytometry（Non-apoptosis/cycle）; Clinical Sample Validation; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
circNUP214 could potentially differentiate RA patients from healthy subjects (AUC = 0.76). |
| Description: |
circNUP214 is up-regulated in PBMCs from rheumatoid arthritis patients and is mainly cytoplasmic. It promotes Th17/IL-17A responses by regulating IL-23R, at least partly by acting as a ceRNA sponge for miR-125a-3p, and shows potential diagnostic value by ROC analysis. |
| Confidence score: |
0.8195 |