| Expression pattern: |
UP |
| Associated gene: |
CIRBP, SRSF3 |
| Associated microRNA: |
- |
| Biological function: |
promotes apoptosis and tubular cell death in iodinated contrast-induced kidney injury |
| Molecular mechanism: |
circSAMD4 binds CIRBP and inhibits its nuclear import, promoting cytoplasmic accumulation of CIRBP and apoptosis; circSAMD4 biogenesis is negatively regulated by SRSF3. |
| Biological pathway or process: |
apoptosis (promotes); other pathway/process (other) |
| Detected method: |
Q
H
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; FISH / smFISH; RIP (RNA Immunoprecipitation); Transfection; Annexin V/PI Flow Cytometry; Western Blot; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; In Vivo Animal Model |
| Clinical significance: |
circSAMD4 expression correlated positively with kidney dysfunction in biopsy-confirmed ATI patients |
| Description: |
circSAMD4 is up-regulated in iodinated contrast-induced kidney injury and promotes renal tubular epithelial cell apoptosis. It binds CIRBP and inhibits its nuclear import, driving CIRBP cytoplasmic accumulation and pro-apoptotic signaling, while SRSF3 downregulation contributes to circSAMD4 upregulation. |
| Confidence score: |
0.7698 |