| Expression pattern: |
UP |
| Associated gene: |
FUS, PRMT1, LAMP1, VHL mRNA, eIF2alpha, HIF-1alpha, G3BP1, FMRP, P62, LC3B, Atg-5, Atg7, Atg12, Ran |
| Associated microRNA: |
- |
| Biological function: |
Promotes TNBC progression by enhancing proliferation, migration/invasion, stress granule dynamics, and lysosome-associated autophagy flux under hypoxic conditions; stabilizes PRMT1 and increases HIF-1alpha via inhibiting VHL translation. |
| Molecular mechanism: |
circTBC1D14 interacts with FUS to promote circRNA biogenesis and forms hypoxia-induced FUS-circTBC1D14 stress granules; circTBC1D14 binds PRMT1 and inhibits K48-linked polyubiquitination to stabilize PRMT1; recruits LAMP1 and reinforces FUS-LAMP1 interaction to enhance lysosome-associated autophagy flux; co-localizes VHL mRNA in SGs to inhibit VHL translation, increasing HIF-1alpha. |
| Biological pathway or process: |
autophagy (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); ubiquitination (inhibits); HIF-1 signaling (promotes); mitochondrial function (other); other pathway/process (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; RIP (RNA Immunoprecipitation); RNA Pull-Down; Western Blot; Co-IP; Luciferase Reporter Assay; Transfection; MTT; EdU Staining; Colony Formation Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Survival Analysis; Bioinformatics Analysis; circRNA-seq |
| Clinical significance: |
High circTBC1D14 expression is associated with poor prognosis and predicts poor overall survival in breast cancer/TNBC; circTBC1D14 could be considered as an independent prognostic factor for overall survival of patients. |
| Description: |
circTBC1D14 is up-regulated in TNBC and promotes tumor growth and metastasis, particularly under hypoxia. Mechanistically, it forms hypoxia-induced stress granules with FUS and promotes lysosome-associated autophagy by recruiting LAMP1, while stabilizing PRMT1 via inhibition of K48-linked polyubiquitination and enhancing hypoxia signaling through the VHL/HIF-1alpha axis. |
| Confidence score: |
0.8856 |