circRNA basic information
circBase ID: -
Name: hsa_circ_PVT1
Synonym: circRNA PVT1 / circPVT1
Host Gene: PVT1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0001056
MONDO name: gastric cancer
Disease details: gastric cancer / GC
Disease DO ID:
 10534
Disease MeSH ID:
 -
Disease NCIt ID:
 C9331
Disease ICD11 ID:
 1397617262
Disease OMIM ID:
 613659
Species: Human
Species details: Homo sapiens
Tissue specimen:

-
Cell lines:

GES-1; AGS; SGC-7901; HEK-293T
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: SMAD3
Associated microRNA: miR-423-5p
Biological function: Promotes gastric cancer cell viability/growth, migration and invasion; promotes EMT in vitro.
Molecular mechanism: Acts as a ceRNA sponging miR-423-5p to upregulate SMAD3 and activate Smad3 signaling, thereby promoting EMT and malignant phenotypes in gastric cancer cells.
Biological pathway or process:

EMT (promotes); migration (promotes); invasion (promotes); proliferation (promotes); ceRNA regulation (promotes); TGF-beta/SMAD (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; CCK8; Transwell Assay; Western Blot; IF (Immunofluorescence)
Clinical significance:

-
Description:

CircPVT1 is up-regulated in gastric cancer cell lines and promotes viability, migration, invasion and EMT. Mechanistically, it acts as a ceRNA that sponges miR-423-5p to relieve repression of SMAD3, increasing Smad3 phosphorylation and activating Smad3 signaling.
Confidence score:

0.5607
Other information
Title:

CircRNA PVT1 modulated cell migration and invasion through Epithelial-Mesenchymal Transition (EMT) mediation in gastric cancer through miR-423-5p/Smad3 pathway.

Journal: Regenerative therapy
Published: 2022
PubMed ID: 35663842
Study type:

biological research
Data availability: Supplementary data to this article can be found online at https://doi.org/10.1016/j.reth.2022.02.003.
Code availability: -