| Expression pattern: |
UN |
| Associated gene: |
ERbeta, ATP2B1, FN1 / fibronectin 1 |
| Associated microRNA: |
miR-204-3p |
| Biological function: |
circATP2B1 suppresses ccRCC cell invasion and metastasis; loss of circATP2B1 reverses the anti-invasive and anti-metastatic effects of ERbeta knockdown, while circATP2B1 overexpression blocks ERbeta-promoted invasion. |
| Molecular mechanism: |
ERbeta suppresses circATP2B1 transcription through binding the ATP2B1 promoter; circATP2B1 acts as a miR-204-3p reservoir to stabilize miR-204-3p, leading to repression of FN1 expression and reduced ccRCC invasion. |
| Biological pathway or process: |
invasion (inhibits); metastasis (inhibits); ceRNA regulation (other); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Bioinformatics Analysis; Transfection; Transwell Assay; RNA Pull-Down; Actinomycin D / DRB Stability Assay; ChIP / ChIP-seq; Luciferase Reporter Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry) |
| Clinical significance: |
The study suggests that therapeutic targeting of the ERbeta/circATP2B1/miR-204-3p/FN1 pathway may help prevent ccRCC progression; no direct circATP2B1 patient prognostic or diagnostic analysis was reported. |
| Description: |
circATP2B1 is a suppressive circRNA in the ERbeta-driven ccRCC invasion pathway. ERbeta represses circATP2B1 through the ATP2B1 promoter, and reduced circATP2B1 decreases miR-204-3p stability, thereby increasing FN1 expression and promoting invasion and metastasis. |
| Confidence score: |
0.7754 |