circRNA basic information
circBase ID: hsa_circ_0026352
Name: hsa_circ_NR4A1
Synonym: circNR4A1
Host Gene: NＲ4A1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007254
MONDO name: breast cancer
Disease details: breast cancer / BC
Disease DO ID:
 1612
Disease MeSH ID:
 -
Disease NCIt ID:
 C9335
Disease ICD11 ID:
 1047754165
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

breast cancer tissues; adjacent normal tissues; peripheral blood
Cell lines:

MCF-7; HCC-1937; MDA-MB-231; T-47D; MCF-10A; HEK293T
In vivo animal model:

-
circRNA-disease information
Expression pattern:
DN
Associated gene: EIF4A3, FUS, K-ＲAS, MEK, EＲK1, Bcl2, BAD, caspase-3
Associated microRNA: miＲ-125, miＲ-138, miＲ-149
Biological function: Inhibits MCF-7 cell proliferation and colony formation, blocks the cell cycle at G1/S phase, and promotes apoptosis.
Molecular mechanism: circNＲ4A1 overexpression suppresses K-ＲAS/MEK/EＲK and Bcl2 expression while increasing BAD and caspase-3 expression; predicted mechanisms include microRNA binding and RNA-binding protein binding.
Biological pathway or process:

ERK (inhibits); apoptosis (promotes); proliferation (inhibits); cell cycle (inhibits); ceRNA regulation (other)
Detected method:
Q
M
Validation methods:

Microarray; RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Western Blot; Bioinformatics Analysis
Clinical significance:

circNＲ4A1 has potential as a molecular diagnostic biomarker and a new molecular therapeutic target for breast cancer.
Description:

circNＲ4A1/Hsa-circ-0026352 is down-regulated in breast cancer tissues, patient peripheral blood, and breast cancer cell lines. Its overexpression in MCF-7 cells suppresses proliferation and colony formation, arrests the cell cycle at G1/S, and promotes apoptosis, likely involving inhibition of the K-RAS/MEK/ERK pathway and modulation of apoptosis-related proteins. The study suggests circNＲ4A1 may serve as a diagnostic biomarker and potential therapeutic target in breast cancer.
Confidence score:

0.697
Other information
Title:

[Over-expression of circNR4A1/Hsa-circ-0026352 inhibits proliferation and induces apoptosis in MCF-7 cells].

Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
Published: 2020
PubMed ID: 33325363
Study type:

combined biological and clinical study
Data availability: -
Code availability: -