| Expression pattern: |
DN |
| Associated gene: |
PAK3 |
| Associated microRNA: |
miR-1252 |
| Biological function: |
inhibits gastric cancer cell viability, proliferation and migration; induces apoptosis and cell cycle arrest; suppresses tumor growth in vivo |
| Molecular mechanism: |
Acts as a sponge of miR-1252 to increase PAK3 expression; miR-1252 mimics block circ_0000190 effects and PAK3 overexpression rescues |
| Biological pathway or process: |
proliferation (inhibits); apoptosis (promotes); cell cycle (inhibits); migration (inhibits); invasion (inhibits); EMT (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
H
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RT-qPCR; FISH / smFISH; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Transwell Assay; Wound Healing Assay; TUNEL; Western Blot; IF (Immunofluorescence); IHC (Immunohistochemistry); In Vivo Animal Model; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
circ_0000190 expression was associated with the overall survival of gastric cancer patients; suggested as a potential prognosis biomarker |
| Description: |
circ_0000190 is down-regulated in gastric cancer and mainly localized in the cytoplasm. Its overexpression suppresses gastric cancer cell growth and motility and induces apoptosis and G1 arrest, acting via a ceRNA axis where circ_0000190 sponges miR-1252 to de-repress PAK3; miR-1252 mimics attenuate these effects and PAK3 re-expression rescues them. |
| Confidence score: |
0.8355 |