| Expression pattern: |
DN |
| Associated gene: |
PTEN, AKT, p-AKT |
| Associated microRNA: |
miR-23a-3p |
| Biological function: |
inhibits proliferation, G1/S transition, and invasion; promotes apoptosis; suppresses tumor growth in vivo |
| Molecular mechanism: |
Acts as a ceRNA (miRNA sponge) for miR-23a-3p to derepress PTEN and inhibit AKT activation (PTEN/Akt pathway). |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (inhibits); invasion (inhibits); apoptosis (promotes); cell cycle (inhibits); PI3K/AKT (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Sanger Sequencing; FISH / smFISH; Luciferase Reporter Assay; Transfection; CCK8; Transwell Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Western Blot; Survival Analysis; Clinical Sample Validation; In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
Low expression of circRHOBTB3 was significantly associated with lymphatic metastasis, and age; circRHOBTB3 was proved to have promising value as a prognosis biomarker. |
| Description: |
circRHOBTB3 (hsa_circ_0007444), derived from RHOBTB3, is down-regulated in epithelial ovarian cancer and acts as a tumor suppressor. It sponges miR-23a-3p to restore PTEN expression and inhibit AKT activation, thereby suppressing proliferation, G1/S transition and invasion while promoting apoptosis, and it also reduces tumor growth in xenograft models. |
| Confidence score: |
0.8174 |