| Expression pattern: |
UP |
| Associated gene: |
CDK19, Ago2, BCL-2, CCND1, PCNA, CDH1, CDH2, VIMENTIN, MMP7 |
| Associated microRNA: |
hsa-miR-205-5p |
| Biological function: |
Promotes NSCLC progression by enhancing proliferation, migration, invasion, metastasis and EMT; affects S-phase cell cycle distribution and anti-apoptosis/proliferation-associated gene expression. |
| Molecular mechanism: |
Acts as a miR-205-5p sponge to up-regulate CDK19, promoting malignant phenotypes and modulating EMT-related markers. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); cell cycle (other); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RT-qPCR; FISH / smFISH; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; MTT; Colony Formation Assay; Cell Cycle Assay; Transwell Assay; Wound Healing Assay; Western Blot; In Vivo Animal Model; Bioinformatics Analysis; Clinical Sample Validation |
| Clinical significance: |
Over-expressed in 60 NSCLC specimens and positively correlated with TNM stage, tumor size, and invasion of the lung basal layer; proposed as a prognostic biomarker. |
| Description: |
hsa_circ_0006692 is up-regulated in NSCLC and functions as an oncogenic circRNA that promotes proliferation, migration/invasion and in vivo metastasis-like spread. Mechanistically, it sponges hsa-miR-205-5p to de-repress CDK19 and modulates EMT marker expression. |
| Confidence score: |
0.8279 |