| Expression pattern: |
UP |
| Associated gene: |
YAP1, MST1, LAST1, YAP, TAZ |
| Associated microRNA: |
- |
| Biological function: |
promotes proliferation; promotes migration; promotes invasion; promotes tumor growth; promotes metastasis |
| Molecular mechanism: |
circPPP1R12A carries an ORF and encodes a 73-aa peptide (circPPP1R12A-73aa) that activates Hippo-YAP signaling to promote colon cancer growth and metastasis. |
| Biological pathway or process: |
Hippo/YAP (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes) |
| Detected method: |
Q
H
M
|
| Validation methods: |
Microarray; RT-qPCR; RNase R Treatment; Back-Splice Junction PCR / divergent primers PCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; ISH (In Situ Hybridization); Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Cell Cycle Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; RNA-seq; Western Blot; Survival Analysis; Cohort Study |
| Clinical significance: |
Patients with higher expression of circPPP1R12A had a shorter overall survival; circPPP1R12A ISH score 3-4 was an independent poor prognostic factor. |
| Description: |
circPPP1R12A (hsa_circ_0000423), derived from PPP1R12A, is up-regulated in colon cancer tissues/cell lines and mainly localized in the cytoplasm. It contains an ORF that encodes a 73-aa peptide (circPPP1R12A-73aa), which promotes colon cancer proliferation and metastasis by activating Hippo/YAP signaling, and higher circPPP1R12A levels are associated with worse overall survival. |
| Confidence score: |
0.7987 |