| Expression pattern: |
UP |
| Associated gene: |
ZEB2, AGO2, RISC complex |
| Associated microRNA: |
miR-138-5p |
| Biological function: |
Promotes gastric cancer cell proliferation, invasion, tumorigenesis, distant metastasis, and aggressive phenotypes. |
| Molecular mechanism: |
circTNIK acts as a ceRNA/molecular sponge for miR-138-5p to regulate ZEB2 expression and promote gastric cancer aggressiveness. |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); migration (promotes); metastasis (promotes); EMT (promotes); ceRNA regulation (other) |
| Detected method: |
Q
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; Microarray; FISH / smFISH; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; In Vivo Animal Model; H&E Staining; Western Blot; Cohort Study; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
Higher circTNIK expression was correlated with aggressive tumor phenotypes and poor overall survival; circTNIK might be used as a prognostic biomarker in gastric cancer patients. |
| Description: |
circTNIK is a TNIK-derived circular RNA that is upregulated in gastric cancer tissues and cell lines, especially in metastatic disease. It promotes gastric cancer proliferation, invasion, tumorigenesis, and lung/liver metastasis by acting as a miR-138-5p sponge to increase ZEB2 expression. High circTNIK expression is associated with aggressive phenotypes and poor overall survival, suggesting prognostic value. |
| Confidence score: |
0.8912 |