| Expression pattern: |
DN |
| Associated gene: |
LPP |
| Associated microRNA: |
miR-223 |
| Biological function: |
Inhibits proliferation, migration and invasion; promotes apoptosis; suppresses tumor growth in xenografts. |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-223, relieving miR-223-mediated repression of LPP (ceRNA-like regulation). |
| Biological pathway or process: |
proliferation (inhibits); migration (inhibits); invasion (inhibits); apoptosis (promotes); ceRNA regulation (other) |
| Detected method: |
Q
M
|
| Validation methods: |
Bioinformatics Analysis; Microarray; divergent primers PCR; Back-Splice Junction PCR; RNase R Treatment; RT-qPCR; FISH / smFISH; Luciferase Reporter Assay; Transfection; MTT; EdU Staining; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Western Blot; In Vivo Animal Model |
| Clinical significance: |
circLRCH3 expression correlated with clinicopathologic indicators (tumor size and TNM stage) and may serve as a diagnostic and therapeutic target for CRC. |
| Description: |
In colorectal cancer, circLRCH3 is down-regulated and functions as a tumor suppressor. It sponges miR-223 to relieve repression of LPP, thereby inhibiting proliferation/migration/invasion and promoting apoptosis, and suppressing xenograft tumor growth. |
| Confidence score: |
0.7566 |