| Expression pattern: |
UP |
| Associated gene: |
COL4A1, Ago2 |
| Associated microRNA: |
miR-1270 |
| Biological function: |
circ_0088364 promotes HCC cell proliferation, cell cycle entrance, migration, invasion, EMT and xenograft tumor growth, and inhibits apoptosis; silencing circ_0088364 suppresses HCC cell growth and motility. |
| Molecular mechanism: |
circ_0088364 functions through a ceRNA mechanism by sponging miR-1270 to regulate COL4A1 expression in HCC. |
| Biological pathway or process: |
proliferation (promotes); cell cycle (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); EMT (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; CCK8; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; In Vivo Animal Model |
| Clinical significance: |
circ_0088364-miR-1270-COL4A1 ceRNA pathway might be a potential therapeutic target to interfere the progression of HCC. |
| Description: |
hsa_circ_0088364/circ_0088364 is upregulated in HCC tumors and HCC cell lines. It acts as an oncogenic circRNA that promotes proliferation, cell cycle progression, migration, invasion, EMT and xenograft tumor growth while suppressing apoptosis, mainly through the circ_0088364-miR-1270-COL4A1 ceRNA axis. Its knockdown suppresses HCC growth and motility, suggesting potential therapeutic relevance. |
| Confidence score: |
0.7294 |