| Expression pattern: |
UP |
| Associated gene: |
HuR, GLUD1 mRNA |
| Associated microRNA: |
- |
| Biological function: |
Promotes proliferation and inhibits apoptosis by suppressing autophagy; supports xenograft tumor growth. |
| Molecular mechanism: |
circTICRR binds the RBP HuR (via F287/F289 in HuR RRM3), enhancing HuR-mediated stabilization of GLUD1 mRNA, increasing GLUD1 protein and thereby inhibiting autophagy. |
| Biological pathway or process: |
autophagy (inhibits); apoptosis (inhibits); proliferation (promotes) |
| Detected method: |
Q
B
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; Northern Blot; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; Annexin V/PI Flow Cytometry; Western Blot; RNA Pull-Down; RIP (RNA Immunoprecipitation); In Vivo Animal Model; TUNEL; H&E Staining; Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
circTICRR (hsa_circ_0036730) is up-regulated in cervical cancer and acts oncogenically by promoting proliferation and inhibiting apoptosis through suppression of autophagy. Mechanistically, circTICRR binds the RBP HuR (F287/F289 in HuR RRM3), enhances HuR recruitment to GLUD1 mRNA, stabilizes GLUD1 mRNA, and elevates GLUD1 protein to inhibit autophagic flux. circTICRR knockdown or a peptide blocking circTICRR-HuR interaction suppresses cell line-derived xenograft growth. |
| Confidence score: |
0.8456 |