| Expression pattern: |
UN |
| Associated gene: |
Smad5, GATA4, AGO2 |
| Associated microRNA: |
miR-145a-5p |
| Biological function: |
promotes angiogenesis; promotes CMEC proliferation; promotes CMEC migration; promotes tube formation; improves heart repair after MI |
| Molecular mechanism: |
GATA4 promotes circERBB2IP biogenesis; circERBB2IP acts as a miRNA sponge for miR-145a-5p to relieve repression on Smad5, increasing Smad5 expression and promoting angiogenesis |
| Biological pathway or process: |
angiogenesis (promotes); proliferation (promotes); migration (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Nuclear-Cytoplasmic Fractionation; Transfection; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Western Blot; EdU Staining; Cell Cycle Assay; Transwell Assay; Tube Formation Assay; IF (Immunofluorescence); In Vivo Animal Model; H&E Staining |
| Clinical significance: |
potential therapeutic target for the treatment of myocardial infarction |
| Description: |
In mouse myocardial infarction models and cardiac microvascular endothelial cells, circERBB2IP promotes post-infarction revascularization and endothelial proliferation/migration/tube formation. It is induced by GATA4 and acts as a ceRNA by sponging miR-145a-5p to increase Smad5, thereby enhancing angiogenesis and improving cardiac repair. |
| Confidence score: |
0.7769 |