| Expression pattern: |
UP |
| Associated gene: |
HuR, SFPQ mRNA, SFPQ, TNF signaling pathway |
| Associated microRNA: |
- |
| Biological function: |
circHMGCS1 promotes NSCLC cell proliferation and migration and promotes tumor growth in vivo. |
| Molecular mechanism: |
circHMGCS1 interacts with the RBP HuR, inhibits HuR ubiquitination and degradation, stabilizes HuR-induced SFPQ mRNA, and regulates the SFPQ/TNF signaling pathway. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); mRNA stability (promotes); ubiquitination (inhibits); apoptosis (inhibits); other pathway/process (inhibits) |
| Detected method: |
Q
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; Microarray; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Co-IP; Luciferase Reporter Assay; Transfection; CCK8; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; Bioinformatics Analysis; RNA-seq |
| Clinical significance: |
circHMGCS1 has potential as a diagnostic and prognostic biomarker and as a potential therapeutic target for NSCLC. |
| Description: |
circHMGCS1 (hsa_circ_0072387) is upregulated in NSCLC tissues and cells. It promotes NSCLC proliferation, migration, and xenograft tumor growth by binding HuR, inhibiting HuR ubiquitin-mediated degradation, and enhancing HuR-mediated stabilization of SFPQ mRNA, with downstream involvement of the TNF signaling pathway. |
| Confidence score: |
0.8866 |