circRNA basic information
circBase ID: hsa_circ_0001322
Name: hsa_circ_EIF4E3
Synonym: circ1322
Host Gene: EIF4E3
Genomic location(hg19): chr3:71733722-71759635:-
Genomic location(hg38): chr3:71684571-71710484:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0001056
MONDO name: gastric cancer
Disease details: gastric cancer
Disease DO ID:
10534
Disease MeSH ID:
-
Disease NCIt ID:
C9331
Disease ICD11 ID:
1397617262
Disease OMIM ID:
613659
Species: Human
Species details: Homo sapiens
Tissue specimen:

GC tissue; paracancerous tissue (normal); gastric cancer tissues; adjacent tissues

Cell lines:

HGC-27; MKN-45; NCl-N87; AGS; SUN-1; GES-1

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
DN
Associated gene: QKI
Associated microRNA: miR-1264
Biological function: circ1322 inhibits gastric cancer cell proliferation, migration, invasion and tumor growth, while promoting apoptosis.
Molecular mechanism: circ1322 acts as a competing endogenous RNA sponge for miR-1264, relieves miR-1264-mediated inhibition of QKI, and suppresses the Hedgehog pathway.
Biological pathway or process:

Hedgehog (inhibits); proliferation (inhibits); migration (inhibits); invasion (inhibits); apoptosis (promotes); ceRNA regulation (promotes)

Detected method:
Q
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; In Vivo Animal Model; Western Blot

Clinical significance:

Low expression of circ_0001322 was negatively correlated with lymph node metastasis, showed diagnostic potential for GC metastasis, and hsa_circ_0001322 may serve as a biomarker for predicting GC prognosis.

Description:

hsa_circ_0001322/circ1322 is down-regulated in gastric cancer tissues and cell lines. Its upregulation suppresses GC proliferation, migration, invasion and xenograft tumor growth while promoting apoptosis. Mechanistically, circ1322 functions as a ceRNA sponge for miR-1264, increases QKI expression, and suppresses Hedgehog pathway activity, suggesting potential biomarker value in GC.

Confidence score:

0.7595

Other information
Title:

Circular RNA Circ_0001322 inhibits gastric cancer progression by modulating the miR-1264/QKI axis and suppressing the Hedgehog pathway.

Journal: Cellular and molecular biology (Noisy-le-Grand, France)
Published: 2024
PubMed ID: 38814217
Study type:

combined biological and clinical study

Data availability: All data generated or analyzed during this study are included in this manuscript.
Code availability: -