| Expression pattern: |
DN |
| Associated gene: |
AU-rich element RNA-binding protein 1 (AUF1), heterogeneous nuclear ribonucleoprotein D (hnRNP D), c-MYC 3 0 -UTR, c-MYC |
| Associated microRNA: |
- |
| Biological function: |
Circ_0000826 inhibits CRC cell proliferation in vitro and in vivo; silencing Circ_0000826 promotes CRC cell proliferation, whereas overexpression restricts proliferation. |
| Molecular mechanism: |
Circ_0000826 binds AUF1 and competitively inhibits AUF1 binding to the c-MYC 3 0 -UTR, thereby reducing c-MYC expression and CRC cell proliferation. |
| Biological pathway or process: |
proliferation (inhibits); other pathway/process (inhibits) |
| Detected method: |
Q
H
S
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; FISH / smFISH; ISH (In Situ Hybridization); Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RNA Pull-Down; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Western Blot; Survival Analysis; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
Circ_0000826 is significantly down-regulated in CRC tissues, associated with poor prognosis, and shows diagnostic potential with an AUC value of 0.823. |
| Description: |
Circ_0000826 is down-regulated in colorectal cancer tissues and low expression is associated with worse patient prognosis. Functionally, Circ_0000826 suppresses CRC cell proliferation in vitro and in a xenograft model. Mechanistically, it binds the RNA-binding protein AUF1 and competes with the c-MYC mRNA 3 0 -UTR for AUF1 binding, thereby reducing c-MYC protein expression and tumor proliferation. |
| Confidence score: |
0.8939 |