| Expression pattern: |
UP |
| Associated gene: |
clec7a |
| Associated microRNA: |
hsa-miRNA-27a-3p, hsa-miRNA-27b-3p, hsa-miRNA-221-5p, hsa-miRNA-1-3p |
| Biological function: |
Suppresses breast cancer/TNBC cell proliferation but promotes cell migration; functions as a sensor/marker of cellular response to CAP treatment. |
| Molecular mechanism: |
Computationally constructed circRNA-miRNA-mRNA network with miRNA hub hsa-miRNA-1-3p; proposed to inhibit RAS-PI3K/AKT and RAS-MAPK signaling to suppress proliferation and to promote p65 phosphorylation to enhance migration. |
| Biological pathway or process: |
PI3K/AKT (inhibits); MAPK (inhibits); proliferation (inhibits); migration (promotes); ceRNA regulation (other) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Bioinformatics Analysis; Transfection; MTT; Wound Healing Assay; Western Blot |
| Clinical significance: |
Proposed as a prognostic marker of tumors feasible for receiving CAP treatment / a sensor of CAP treatment response. |
| Description: |
hsa_circRNA_0040462 is induced by CAP treatment in breast cancer cells and serves as a sensitive marker of CAP response. Functionally, it shows double-edged effects in TNBC cells: it restrains proliferation but enhances migratory ability, potentially through a circRNA-miRNA-mRNA network (hub hsa-miRNA-1-3p) and modulation of PI3K/AKT and MAPK signaling and p65 phosphorylation. |
| Confidence score: |
0.5008 |