| Expression pattern: |
UP |
| Associated gene: |
SORT1, Beclin-1, LC3B, p62 |
| Associated microRNA: |
miR-127-5p, miR-136-5p |
| Biological function: |
promotes proliferation, migration, and invasion of gastric cancer cells; promotes tumor growth in vivo; promotes autophagy |
| Molecular mechanism: |
Acts as a ceRNA/miRNA sponge for miR-127-5p and miR-136-5p to upregulate SORT1 (primarily at protein/translation level), thereby promoting gastric cancer progression and autophagy. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); autophagy (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); IF (Immunofluorescence); Western Blot; Clinical Sample Validation; Cohort Study; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High hsa_circ_0110389 expression associates with advanced stages of GC and predicts poor prognosis; independent prognostic factor for OS and DFS. |
| Description: |
hsa_circ_0110389 is up-regulated in gastric cancer tissues/cells and is mainly cytoplasmic. It promotes GC proliferation, migration, invasion and xenograft growth by sponging miR-127-5p and miR-136-5p, thereby increasing SORT1 expression and influencing autophagy-related proteins. |
| Confidence score: |
0.8847 |