circRNA basic information
circBase ID: hsa_circ_0007255
Name: hsa_circ_KIF4A
Synonym: circKIF4A / hsa_circRNA_0007255
Host Gene: KIF4A
Genomic location(hg19): chrX:69549254-69553539:+
Genomic location(hg38): chrX:70329404-70333689:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0009807
MONDO name: osteosarcoma
Disease details: osteosarcoma / OS
Disease DO ID:
 3347
Disease MeSH ID:
 D012516
Disease NCIt ID:
 C9145
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

OS tissues; non-cancerous bone tissues
Cell lines:

SOSP-9607; HOS; U2OS; SW1353; Saos-2; hFOB1.19
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: SLC7A11, Ago2
Associated microRNA: miR-515-5p
Biological function: promotes osteosarcoma proliferation and metastasis
Molecular mechanism: Acts as a ceRNA sponge for miR-515-5p to upregulate SLC7A11 (AGO2/RISC-associated).
Biological pathway or process:

proliferation (promotes); metastasis (promotes); invasion (promotes); ceRNA regulation (promotes); ferroptosis (inhibits)
Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Transfection; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; CCK8; Colony Formation Assay; Transwell Assay; In Vivo Animal Model; H&E Staining; Western Blot; Bioinformatics Analysis; Clinical Sample Validation
Clinical significance:

potential prognostic implications for OS; important in OS monitoring and treatment
Description:

circKIF4A is up-regulated in osteosarcoma tissues and cell lines and promotes OS proliferation, invasion, and lung metastasis. Mechanistically, it functions as a ceRNA that sponges miR-515-5p, relieving repression of SLC7A11 (AGO2/RISC-associated), with effects linked to ferroptosis-related redox status (GSH/GSSG).
Confidence score:

0.8278
Other information
Title:

CircKIF4A enhances osteosarcoma proliferation and metastasis by sponging MiR-515-5p and upregulating SLC7A11.

Journal: Molecular biology reports
Published: 2022
PubMed ID: 35579738
Study type:

combined biological and clinical study
Data availability: Data will be made available if needed.
Code availability: -