| Expression pattern: |
UP |
| Associated gene: |
EPSTI1, Ago2 |
| Associated microRNA: |
miR-942 |
| Biological function: |
promotes ovarian cancer cell proliferation and invasion and inhibits apoptosis |
| Molecular mechanism: |
circEPSTI1 functions as a ceRNA/miRNA sponge to decoy miR-942 and thereby regulate EPSTI1 expression; both are recruited to an Ago2-related RISC. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); invasion (promotes); apoptosis (inhibits); metastasis (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; Annexin V/PI Flow Cytometry; Transwell Assay; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Western Blot; In Vivo Animal Model; H&E Staining; Bioinformatics Analysis |
| Clinical significance: |
circEPSTI1 could be used as a biomarker and therapeutic target in ovarian cancer. |
| Description: |
circEPSTI1 is up-regulated in ovarian cancer tissues and promotes malignant phenotypes (proliferation, invasion, tumor growth/metastasis) while inhibiting apoptosis. Mechanistically, it mainly localizes to the cytoplasm and acts as a ceRNA that sponges miR-942 to upregulate EPSTI1. |
| Confidence score: |
0.73 |