circRNA basic information
circBase ID: hsa_circ_0008826
Name: hsa_circ_DHTKD1
Synonym: circDHTKD1-475aa
Host Gene: DHTKD1
Genomic location(hg19): chr10:12136071-12162266:+
Genomic location(hg38): chr10:12094072-12120267:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer / CRC
Disease DO ID:
 5672, 9256
Disease MeSH ID:
 -
Disease NCIt ID:
 C4978
Disease ICD11 ID:
 -
Disease OMIM ID:
 114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

human solid tissues
Cell lines:

DLD-1; HCT116; SW480; SW620; Lovo; HT29; CACO2; NCM460
In vivo animal model:

-
circRNA-disease information
Expression pattern:
DS
Associated gene: DHTKD1
Associated microRNA: -
Biological function: Promotes proliferation, colony formation and invasion of CRC cells; minor effect on cell cycle and no effect on apoptosis (as reported upon silencing).
Molecular mechanism: Peptide-coding circRNA; encoded peptide detected (circDHTKD1-475aa). Downstream mechanism not defined.
Biological pathway or process:

proliferation (promotes); invasion (promotes)
Detected method:
Q
M
Validation methods:

Microarray; RT-qPCR; Clinical Sample Validation; Sanger Sequencing; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; Bioinformatics Analysis
Clinical significance:

-
Description:

hsa_circ_0008826 (DHTKD1-derived) shows variable expression across CRC cell lines and can encode a peptide (circDHTKD1-475aa). Knockdown in DLD-1 cells inhibits proliferation/colony formation and invasion, with subtle cell-cycle changes and no detectable apoptosis effect.
Confidence score:

0.7421
Other information
Title:

Strategy for Scanning Peptide-Coding Circular RNAs in Colorectal Cancer Based on Bioinformatics Analysis and Experimental Assays.

Journal: Frontiers in cell and developmental biology
Published: 2021
PubMed ID: 35281715
Study type:

combined biological and bioinformatics study
Data availability: GSE126095; GSE142837
Code availability: -