| Expression pattern: |
UP |
| Associated gene: |
ETS1 |
| Associated microRNA: |
miR-338-3p |
| Biological function: |
enhances LPS-induced inflammatory responses and promotes inflammatory cytokine (IL-6, VEGF) production in BEAS-2B cells |
| Molecular mechanism: |
Acts as a ceRNA/miRNA sponge for miR-338-3p to regulate ETS1 and ERK pathway activation |
| Biological pathway or process: |
ERK (promotes); inflammation (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; Transfection; CCK8; ELISA; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
circDHTKD1 may serve as a potential therapeutic target against asthma |
| Description: |
In an LPS-induced in vitro airway epithelial inflammation model relevant to asthma, circDHTKD1 is up-regulated and mainly localized in the cytoplasm. circDHTKD1 promotes inflammatory cytokine production by sponging miR-338-3p, thereby relieving repression of ETS1 and enhancing ERK pathway activation. |
| Confidence score: |
0.7296 |