circRNA basic information
circBase ID: hsa_circ_0000497
Name: hsa_circ_SLAIN1
Synonym: circ_0000497
Host Gene: SLAIN1
Genomic location(hg19): chr13:78293666-78327493:+
Genomic location(hg38): chr13:77719531-77753358:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005140
MONDO name: ovarian carcinoma
Disease details: epithelial ovarian cancer / EOC
Disease DO ID:
 4001
Disease MeSH ID:
 -
Disease NCIt ID:
 C4908
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

primary tumor lesions; tumor cells from ascites; metastatic lesions
Cell lines:

SKOV3; OVCAR3
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UN
Associated gene: ANO8, DSG3, NPHS1, PLAT, RBFOX1, FFAR2, ASB16, CAMK2A, MAPK8IP3, CACNA1I, CARNS1, SLC43A2, FRAS1, TMEM151A, TNFRSF8, STX11, SMPD3, NCCRP1, TSPAN32
Associated microRNA: hsa-miR-1257, hsa-miR-138-2-3p, hsa-miR-200b-3p, hsa-miR-200c-3p, hsa-miR-301a -5p, hsa-miR-3150a-3p, hsa-miR-4691-5p, hsa-miR-6134
Biological function: promotes migration and invasion (metastasis) of EOC cells via EMT in vitro; promotes proliferation
Molecular mechanism: ceRNA network prediction (circRNA sponging miRNAs to regulate downstream mRNAs); EMT marker regulation upon circRNA overexpression/silencing
Biological pathway or process:

EMT (promotes); migration (promotes); invasion (promotes); proliferation (promotes); ceRNA regulation (other)
Detected method:
Q
S
Validation methods:

RNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; Clinical Sample Validation; Transfection; Wound Healing Assay; Transwell Assay; Western Blot
Clinical significance:

may serve as novel potential therapeutic targets or prognostic biomarkers for EOC peritoneal metastasis
Description:

In EOC ascitic metastasis, hsa_circ_0000497 promotes ovarian cancer cell migration and invasion in vitro, consistent with an EMT-promoting role. The study also constructed a predicted circRNA-miRNA-mRNA ceRNA network as a potential mechanism.
Confidence score:

0.7046
Other information
Title:

Hsa_circ_0000497 and hsa_circ_0000918 contributed to peritoneal metastasis of ovarian cancer via ascites.

Journal: Journal of translational medicine
Published: 2022
PubMed ID: 35538537
Study type:

combined biological and clinical study
Data availability: available from the corresponding author on reasonable request
Code availability: -