| Expression pattern: |
UP |
| Associated gene: |
LDHA, FGFR1 |
| Associated microRNA: |
- |
| Biological function: |
promotes aerobic glycolysis and proliferation; promotes tumor growth in vivo |
| Molecular mechanism: |
circVAMP3 directly binds LDHA and enhances LDHA enzymatic activity by facilitating FGFR1-dependent phosphorylation of LDHA at Y10; does not act as a miRNA sponge |
| Biological pathway or process: |
glycolysis (promotes); proliferation (promotes); oxidative phosphorylation (inhibits); other pathway/process (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; Bioinformatics Analysis; RT-qPCR; Clinical Sample Validation; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; FISH / smFISH; Transfection; RNA Pull-Down; RIP (RNA Immunoprecipitation); IF (Immunofluorescence); Western Blot; Co-IP; CCK8; EdU Staining; Colony Formation Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Flow Cytometry（Non-apoptosis/cycle） |
| Clinical significance: |
circVAMP3 expression was elevated in RCC patients in correspondence with TNM stage; circVAMP3 may represent an RCC biomarker and treatment target |
| Description: |
circVAMP3 is upregulated in RCC/ccRCC and correlates with advanced TNM stage. It promotes aerobic glycolysis and proliferation by directly binding LDHA and enhancing FGFR1-dependent LDHA Y10 phosphorylation, increasing LDHA activity and tumor growth in xenografts. |
| Confidence score: |
0.8794 |