circRNA basic information
circBase ID: hsa_circ_0000284
Name: hsa_circ_HIPK3
Synonym: circHIPK3 / circRNA homeodomain interacting protein kinase 3
Host Gene: -
Genomic location(hg19): chr11:33307958-33309057:+
Genomic location(hg38): chr11:33286412-33287511:+
Subcellular localization: unclear
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005180
MONDO name: Parkinson disease
Disease details: Parkinson's disease / PD
Disease DO ID:
 14330
Disease MeSH ID:
 D010300
Disease NCIt ID:
 C26845
Disease ICD11 ID:
 296066191
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

serum; blood samples; cerebral fluid; CSF
Cell lines:

BV2; SH-SY5Y
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: STAT3, NLRP3, CD11b, Iba-1, caspase-1, ASC, IL-6, IL-1beta, TNF-alpha, ROS
Associated microRNA: miR-124 / miR-124-3p
Biological function: promotes neuroinflammation, microglial activation, pyroptosis, inflammatory cytokine secretion, ROS production, and SH-SY5Y cell apoptosis; decreases SH-SY5Y cell viability in PD-related in vitro models
Molecular mechanism: circHIPK3 functions as a miR-124 sponge and regulates the miR-124-mediated STAT3/NLRP3 inflammatory signaling pathway
Biological pathway or process:

inflammation (promotes); immune regulation (promotes); pyroptosis (promotes); apoptosis (promotes); oxidative phosphorylation (other); other pathway/process (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Transfection; MTT; Annexin V/PI Flow Cytometry; ELISA; Western Blot; FISH / smFISH; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); Northern Blot; Bioinformatics Analysis
Clinical significance:

circHIPK3 was increased in serum/blood and CSF of PD patients and could be a potential target in the treatment of PD
Description:

circHIPK3 is up-regulated in serum/blood and CSF from PD patients and in LPS-induced PD-related cell models. It promotes neuroinflammation, microglial activation, pyroptosis, inflammatory cytokine release, and ROS production by sponging miR-124 and activating the STAT3/NLRP3 signaling pathway, suggesting potential therapeutic relevance in PD.
Confidence score:

0.7618
Other information
Title:

CircHIPK3 promotes neuroinflammation through regulation of the miR-124-3p/STAT3/NLRP3 signaling pathway in Parkinson's disease.

Journal: Advances in clinical and experimental medicine : official organ Wroclaw Medical University
Published: 2023
PubMed ID: 36306116
Study type:

combined biological and clinical study
Data availability: https://doi.org/10.5281/zenodo.7074360; Supplementary File; Supplementary Table
Code availability: -