| Expression pattern: |
DN |
| Associated gene: |
DDX5 / DEAD-box helicase 5 / Ago2 |
| Associated microRNA: |
miR-340-5p |
| Biological function: |
Mitigates cerebral ischemia-reperfusion/OGD/R-induced neuronal injury by inhibiting apoptosis and inflammation and reducing LDH release; knockdown aggravates injury and promotes glial activation. |
| Molecular mechanism: |
Acts as a competing endogenous RNA (ceRNA) that sponges miR-340-5p to regulate DDX5, thereby modulating NF-kappaB activation and neuronal injury phenotypes. |
| Biological pathway or process: |
apoptosis (inhibits); inflammation (inhibits); NF-kappaB (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; Annexin V/PI Flow Cytometry; ELISA; Western Blot; TUNEL; IF (Immunofluorescence); In Vivo Animal Model; H&E Staining |
| Clinical significance: |
- |
| Description: |
circPUM1 is down-regulated in tMCAO mouse brain and OGD/R-treated SH-SY5Y cells. Functionally, circPUM1 overexpression alleviates neuronal injury (reduced apoptosis, inflammation, LDH release) by sponging miR-340-5p to increase DDX5 and suppress NF-kappaB pathway activation. |
| Confidence score: |
0.6279 |