| Expression pattern: |
UP |
| Associated gene: |
HMGA2 |
| Associated microRNA: |
miR-766-5p |
| Biological function: |
accelerates proliferation, invasion, angiogenesis, stem cell-like properties and tumor growth in LSCC |
| Molecular mechanism: |
circSHKBP1 acts as a competing endogenous RNA and sponges miR-766-5p to regulate HMGA2 |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); angiogenesis (promotes); stemness (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis; Spearman's rank correlation analysis; Transfection; CCK8; Transwell Assay; Tube Formation Assay; Colony Formation Assay; Luciferase Reporter Assay; RNA Pull-Down; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry) |
| Clinical significance: |
elevated circSHKBP1 expression was closely linked to poor prognosis; patients with high circSHKBP1 expression showed lower overall survival |
| Description: |
circSHKBP1 is up-regulated in LSCC tissues and cell lines and is associated with poorer overall survival. Functionally, circSHKBP1 promotes LSCC proliferation, invasion, angiogenesis, stemness, and xenograft tumor growth by acting as a ceRNA that sponges miR-766-5p to derepress HMGA2. |
| Confidence score: |
0.8534 |