| Expression pattern: |
UP |
| Associated gene: |
ZEB1, Ago2 |
| Associated microRNA: |
miR-485-3p |
| Biological function: |
Circ-DNMT1 promotes breast cancer cell colony formation, migration, invasion and xenograft tumor growth, and inhibits apoptosis; circ-DNMT1 knockdown restrains these malignant phenotypes. |
| Molecular mechanism: |
Circ-DNMT1 acts through a ceRNA-like circ-DNMT1/miR-485-3p/ZEB1 axis: it negatively regulates miR-485-3p and thereby increases ZEB1 expression. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; Colony Formation Assay; Transwell Assay; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
Circ-DNMT1 may act as a promising target for breast cancer development and treatment. |
| Description: |
Circ-DNMT1 is up-regulated in breast cancer tissues and cell lines. Functionally, circ-DNMT1 knockdown suppresses colony formation, migration, invasion and xenograft tumor growth while promoting apoptosis. Mechanistically, circ-DNMT1 regulates breast cancer development through the miR-485-3p/ZEB1 axis. |
| Confidence score: |
0.7977 |